Your advisor has given you a tube of GMP that has been sitting in the freezer for many years and wants to know the concentration. There is one problem: the spectrophotometer in the lab has been 'optimized' for use with proteins and only measures at 280 nm. You decide to give it a try. After getting and absorbance reading of 0.2, what is the concentration of GMP in the tube (assuming a 1 cm path length).

Answers

Answer 1

Answer:

2.22 × 10^-5M

Explanation:

From Beer-Lambert's law;

A=εcl

Where A = absorbance of GMP= 0.2

ε= molar extinction coefficient of GMP = 9000 M-1cm-1

c= concentration of GMP= the unknown

l= path length = 1 cm

c= A/εl = 0.2/ 9000 M-1cm-1 × 1 cm

c= 2.22 × 10^-5M


Related Questions

Which of the following situations best describes an ionic bond?
Two atoms each give up an electron, and an electrostatic force attracts them.
One atom gains an electron while the other atom loses an electron, and an
electrostatic force attracts them.
One atom gains an electron while the other atom loses an electron, and an
electrostatic force repels them.
Two atoms gain electrons and an electrostatic force attracts them.

Answers

One atom gains an electron while the other atom loses an electron and a electrostatic force attracts them.

The situation which best describes the ionic bond is “ One atom gains an electron while the other atom loses an electron, and an electrostatic force attracts them”.

What is an ionic bond?

An ionic bond is formed between two atoms by donating an electron from the electropositive atom and withdrawal of that electron by the second atom which is electronegative and thereby attaining stability by making their valency completed.

The electron loss-gain interaction make an electrostatic force of attraction between them, where the electrons from the electropositive atom is attracted towards the electronegative atom.

Thus the two atoms are bound together by the electrostatic force of attraction created by the electron donation. Hence, option b is correct.

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A field produced by a magnetic object that exerts a force on other magnetic materials or moving electrical charges is called alan a Magnetic field b Electric field Electrostatic field d All of the above​

Answers

Answer: magnetic field

A field produced by a magnetic object that exerts a force on other magnetic materials or moving electrical charges is called a Magnetic field.

What is a magnetic field?

The magnetic subject is the region around a magnet in which the effect of magnetism is felt. We use the magnetic area as a device to describe how the magnetic force is shipped inside the area around and inside something magnetic in nature.

Why magnetic area is important?

The magnetic area is extremely critical to maintaining lifestyles in the world. without it, we'd be exposed to excessive amounts of radiation from the solar, and our environment would be unfastened to leak into space.

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If 2.1 mL of 70.0% alcohol is added to 6.7 mL of water, what is the percent alcohol (%v/v) in the new mixture?

Answers

Answer:

Since we are adding more water to an alcohol solution,

the volume of alcohol in the alcohol will be the same but the volume percent of alcohol will decrease

Initial Volume of Alcohol in the solution:

We are given that 70% of the 2.1 mL solution is alcohol

So, Finding 70% of 2.1

70/100 * 2.1 = 1.470 mL alcohol

Volume of Water in the Solution:

In our initial solution, we have (2.1 - 1.47) mL water

and we add an additional 6.7 mL water in the solution

Hence,

the total amount of water in the solution = (2.1- 1.47) + 6.7

Total amount of water in the solution  = 0.63 + 6.7

Total amount of water in the solution = 7.33 mL

Volume of the final solution = 8.8mL

Percent Alcohol in the new mixture:

Now that we know the volume of alcohol and the volume of water in our mixture, we can now find the percent composition of alcohol

Percent of alcohol (%v/v) = Amount of alcohol / 100mL of solution

Percent of alcohol = (1.47 / 8.8) * 100

Percent of alcohol = 0.167 * 100

Percent of alcohol = 16.7

Therefore, in a 100mL solution, we will have 16.7 mL alcohol

sharpining a pencil leaves behind pencil shavings. Is sharpining a pencil a physical change a physical or chemical? Explain

Answers

Answer:

A physical change

Explanation:

Cause when sharpening a pencil the led stays as led and only changes the height of it not the composition of it

What would happen if I throw nail polish remover into the fireplace

Answers

Answer:

Your house could catch fire because while Nail Polish Remover itself isn't particularly flammable, the vapors it gives off are. Because of this, sparks could occur if nail polish is used near open flame or was tossed in, and then the sparks could catch onto something else and grow. I would not recommend trying this.

Explanation:

What’s it called when a sound wave is slowed down by a new medium?

Answers

Answer:

Refraction of waves involves a change in the direction of waves as they pass from one medium to another. ... Sound waves travel slower in cooler air than they do in warmer air.

Explanation:

How is the term stable used in the text?

Answers

Answer:

When something is stable, it's fixed and steady. If you needed advice, you'd probably go to your most stable friend, the one least likely to act crazy or be easily upset.

Explanation:

Whether you're talking about an object or a person, the adjective stable implies reliability and strength. You can describe a government as stable, or a relationship, or a desk. A completely different meaning of stable is the noun "building used for housing horses or other animals." Both senses of the word come from the Latin stabilis, "firm or steadfast."

Liquid hexane will react with gaseous oxygen to produce gaseous carbon dioxide and gaseous water . Suppose 17. g of hexane is mixed with 101. g of oxygen. Calculate the maximum mass of carbon dioxide that could be produced by the chemical reaction. Be sure your answer has the correct number of significant digits.

Answers

Answer:

52g of carbon dioxide

Explanation:

Liquid hexane + gaseous oxygen --> Carbon dioxide + gaseous water

The balance chemical equation is given as;

C6H12 + 9O2 --> 6CO2 + 6H2O

From the stochiometry of the reaction;

1 mol of hexane (86g) would consume 9 mol of oxygen (144g) to form 4 mol of carbon dioxide (264g)

The limiting reactant in this equation is hexane as it would run out before oxygen. This means hexane determines how much of the carbon dioxide is formed.

86g of hexane = 264g of crbon dioxide

17g of hexane = xg of carbon dioxide

x = 17 * 264 / 86 =  52g of carbon dioxide

20 POINTS A group of students is conducting a density experiment with two blocks. Block 1 has more mass and less volume than Block 2. Based on this information, the student can correctly conclude that *


both blocks have densities less than water's density

both blocks have densities greater than water's density

Block 1 has a higher density than Block 2

Block 1 has a lower density than Block 2

Answers

Answer:

Block 1 has a higher density then block 2

Explanation:

Answer:

Yep, the correct answer is C. Block 1 has a higher density than block 2.

Explanation:

How many total atoms are in 0.550 g of P2O5?

Answers

Answer:

Total atoms in 0.550g of =1.631x1022

Explanation:

answer is above

Which element is a gas?

A.Br
B.C
C.B
D.Be

Answers

The answer should be (B) C

Answer:

A.Br

Explanation:

Bromine is the only gas from the choices provided. It belongs to the group of halogens, group 7.

It has atomic number of 35 and it belongs to the p-block on the periodic table.

C is carbon and carbon is a solid matter B is boron and boron is a metalloid Be is beryllium and silvery white metal

Only Bromine is the gas from the given choices. It is fuming liquid and when exposed, it becomes gaseous.

9) Which must be a mixture of substances?

A) solid
B) liquid
C) gas
D) solution

Answers

Answer:

D

Explanation:

If two atoms are the same element, which statement can never be true?

1: they can have different number of protons.

2:they can have different number of electrons.

3:they can have different number of neutrons

Answers

Answer:

The answer is number 3: They can have different number of neutrons

Explanation:


Which of the following best describes the change in Antarctic temperature from about 440,000 years ago to about 340,000 years ago?
A
The temperature increases by about 10 S
B
The temperature decreases by about 8°C.
С
The temperature increases by about 12°C and then decreases by about 12°C.
D
The temperature decreases by about 10°C and then increases by about 12°C.

Answers

Answer: С . The temperature increases by about 12°C and then decreases by about 12°C.

Explanation:

Temperatures around the world have been on the rise since the Industrial revolution as humans clog the planet with Carbon Dioxide and other pollutants. This had led to a rise in temperatures that has seen ice levels fall and sea levels rise around the world.

Temperature fluctuations on the other hand are not a new thing. Studies show that in Antarctica temperatures from about 440,000 years ago to about 340,000 years ago increased by 12°C and then decreased by about 12°C.

Answer:

  C.  The temperature increases by about 12°C and then decreases by about 12°C.

Explanation:

The attached shows ice core data that indicates the time period of interest was about the period of one full cycle of climate change. Temperature went up, then went down again to about its original level. The range of change was on the order of 12 °C.

Which is the electron configuration for boron?
A.1s22s3
B.1s22s23s
C.1s'2s²2p?
D.1s22s²2p?

Answers

Answer:

electron configuration for boron is 1s22s22p1

hope this helps :)

Explanation:

Please answer 3 and 2

Answers

Answer:

3 is c but im unable to see the 2nd questions picture

Explanation:

Chemical equation to show cdso4 dissolves in water

Answers

no se bb si quieres salimos tu sabes

A hypothesis must be testable. Which hypothesis is testable? *

Ablue is the best color.
BSummer is nicer than fall.
CDogs are better than cats.
DA beagle can jump higher than a Persian cat.

Answers

the answer is D because can be tested the others ones is opinion

please mark me as brainliest

The water cycle would not be possible without ______ and ________.

Options
1. evaporation; condensation
2.gravity; sun
3.precipitation; rain
4.gravity; precipitation

Answers

Answer:

I think it's 4

Explanation:

but I don't really remember the water cycle

What formations are created by sand deposition by waves?

Cirques
Dunes
Horns
Spits

Answers

Answer:

Spits

Explanation:

Deposit by waves include beaches. They may shift along the shoreline due to longshore drift. Other wave deposits are spits, sandbars and barrier islands

Answer: D

Explanation:

What is the percent yield for the reaction below when
705.0 g SO2 and 80.0 g 02 produce 586.0 g S03?
2802(g) + O2(g) → 2503(9)

Answers

Answer:

Percent yield of reaction is 150%.

Explanation:

Given data:

Percent yield = ?

Actual yield of SO₃ = 586.0 g

Mass of SO₂ = 705.0 g

Mass of O₂ = 80.0 g

Solution:

Chemical equation:

2SO₂ + O₂      →     2SO₃

Number of moles of SO₂:

Number of moles = mass/ molar mass

Number of moles = 586.0 g/ 64.1 g/mol

Number of moles = 9.1 mol

Number of moles of O₂:

Number of moles = mass/ molar mass

Number of moles = 80.0 g/ 32g/mol

Number of moles = 2.5 mol

Now we will compare the mole of SO₃ with O₂ and SO₂.

                      SO₂          :          SO₃

                        2             :            2

                     9.1              :           9.1

                       O₂            :          SO₃

                        1              :            2

                     2.5             :           2×2.5 = 5

The number of moles of SO₃ produced by oxygen are less it will limiting reactant.

Theoretical yield of SO₃:

Mass = number of moles × molar mass

Mass = 5 mol × 80.1 g/mol

Mass = 400.5 g

Percent yield of reaction:

Percent yield = actual yield / theoretical yield  × 100

Percent yield = 586.0 g/ 400.5 g× 100

Percent yield = 1.5× 100

Percent yield = 150%

Fill in the blank... spelling counts.

Speed is the ______ something travels divided by the _____ It takes to travel that dinstance.

Answers

Answer:

[tex]speed = \frac{distance}{time}[/tex]

What does the presence of a polar covalent bond show about the electronegativity of its two atoms

Answers

Answer:

The electronegative of two atoms are not equal.

Explanation:

Is it true or false that the Temperature is a direct measurement of the energy stored in a substance?

Answers

False, Temperature is a physical quantity that expresses hot and cold

how many orbitals are in 6s sublevel

Answers

Each sublevel can have 6 electrons , basically 2 s orbital electrons 6 p orbitals electrons 14 f orbital electrons

Hope this helps.. if not sorry

The azimuthal quantum number and magnetic quantum number corresponds to the s orbital is zero. Hence, there is only one m value and thus contains one orbital only.

What are quantum numbers ?

The quantum numbers are numbers used to describe the position of an electron within an orbital. The principal quantum number n indicates the main energy level. The azimuthal quantum number l indicates the shape and type of orbital which have values from 0 to n-1.

The magnetic quantum number m indicates the number of orbitals for a sublevel. m can have values from + l to -l. The fourth quantum number s is called spin quantum number. For an electron the spin quantum number can be +1/2 or -1/2.

The n value for the 6s sublevel is 6. The azimuthal quantum number , l for an s orbital  is zero. So its m quantum number has only one value that is zero. Hence, there is only one orbital in 6s sublevel.

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